Isolation and characterization of novel α-amylase from marine Streptomyces sp. D1 |
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Authors: | Samrat Chakraborty Abhijit Khopade Chandrakant Kokare Kakasaheb Mahadik Balasaheb Chopade |
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Institution: | aDepartment of Pharmaceutical Biotechnology, Bharati Vidyapeeth University, Poona College of Pharmacy, Erandwane, Pune 411 038, India;bInstitute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, India |
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Abstract: | In this study, we have reported novel α-amylase enzyme from less extensively studied marine Streptomyces sp. D1. Enzyme production was determined by using media containing 2% sucrose, 0.35% peptone and 0.15% of malt extract. Optimum temperature for enzyme production and activity was found to be 45 °C and enzyme retained almost 50% of its activity at 85 °C. Enzyme activity was also retained in presence of commercially available detergent and oxidizing agents. The partially purified enzyme from strain D1 exhibited specific activity of 113.64 U/mg protein that corresponds to 2.8-fold purification. SDS-PAGE and zymogram activity staining showed a single band equal to molecular mass of 66 kDa. The reported enzyme may have wide spread application for detergent and pharmaceutical industry. |
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Keywords: | Marine Streptomyces Halophilic Surfactant Detergent stable |
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