Subsite mapping of Aspergillus niger glucoamylases I and II with malto- and isomaltooligosaccharides |
| |
Authors: | Meagher M M Nikolov Z L Reilly P J |
| |
Affiliation: | Department of Chemical Engineering, Iowa State University, Ames, Iowa 50011. |
| |
Abstract: | Glucoamylase, industrially derived from Aspergillus niger, was chromatographically separated into forms I and II and purified to near homogeneity. Preparations were proved to be free of D-glucosyltransferase by electrophoretic and differential inhibition tests. Maximum rates and Michaelis constants were obtained for both glucoamylases I and II with maltooligosaccharides from maltose to maltoheptaose and with isomaltooligosac-charides from isomaltose to isomaltohexaose. Subsite maps were calculated from these kinetic data and were not significantly different for the two forms. Subsites in both forms had lower affinities for D-glucosyl residues contained in isomaltooligosaccharides than for D-glucosyl residues in maltooligosaccharides. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|