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Pilosulin 5, a novel histamine-releasing peptide of the Australian ant, Myrmecia pilosula (Jack Jumper Ant) |
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| Authors: | Inagaki Hidetoshi Akagi Masaaki Imai Hirotami T Taylor Robert W Wiese Michael D Davies Noel W Kubo Tai |
| Institution: | a Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan b Faculty of Pharmaceutical Sciences, Tokushima Bunri University Yamashiro-cho, Tokushima, Tokushima 770-8514, Japan c Suwabus Bessouchi, K-33 Kitayama, Tateshina, 4035-1674, Chinoshi, Nagano 391-0301, Japan d 28 Ambalindum Street, Hawker, ACT 2614, Australia e Department of Pharmacy, Royal Hobart Hospital, GPO Box 1061L, Hobart 7001, Australia f Sansom Institute, University of South Australia, GPO Box 2471, Adelaide 5001, Australia g Menzies Research Institute, University of Tasmania, Private Bag 23, Hobart 7001, Australia h Central Science Laboratory, University of Tasmania, Private Bag 74, Hobart 7001, Australia |
| Abstract: | Venom of the Australian ant species Myrmecia pilosula contains a number of allergenic peptides including pilosulins. To obtain novel cDNA clones that encode the pilosulin-related bioactive peptides, mRNA of M. pilosula species complex was subjected to RT-PCR in which the forward primer corresponds to a nucleotide sequence in the leader sequences of pilosulins. We isolated a cDNA clone encoding the novel bioactive peptide pilosulin 5. Tandem mass analysis was entirely consistent with the cDNA derived sequence, and indicated that pilosulin 5 is connected by a single disulfide bridge to create a dimmer peptide of 8546 Da. Synthetic pilosulin 5 peptide caused a significant histamine release in a dose-dependent manner, and the mastoparan homologous region of pilosulin 5 was responsible for the activity. |
| Keywords: | Ant Pilosulin Myrmecia pilosula Histamine-releasing activity |
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