Segmental flexibility of Fc-region of spin labeled 2 subclasses of IgG molecule from cow blood and local conformational mobility of their carbohydrate components

Spin-label was bound to carbohydrates of Fc-region of two subclasses of the IgG molecule from the blood serum of healthy and ill cows. By the value of rotational correlation time (10 nsec) internal flexibility of Fc-region of both IgG subclasses and by value of the order parameter (S = 0.9)--rigid attachment of oligosaccharide chains to protein parts of the molecules were found. At the same time the states of IgG1 an IgG2 molecules determined by dynamic steric characteristics for ill and healthy cows did not differ.