Extraction of chlorophyll and carotenoid pigments from Antarctic benthic mats for analysis by HPLC |
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| Authors: | Malcolm T. Downes Liana Hrstich Warwick F. Vincent |
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| Affiliation: | (1) National Institute of Water and Atmospheric Research Ltd. Freshwater, PO Box 8602, Riccarton, Christchurch, New Zealand;(2) Present address: Department de Biologie, Faculté des Sciences et de Genie, Cité Universitaire, Université Laval, Quebec, Canada, GK1 7P4 |
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| Abstract: | The efficiency of 9:1 acetone-water, DMSO and boiling 9:1 ethanol-water in extracting chlorophyll and carotenoid pigments from benthic cyanobacterial mats from Antarctica for HPLC (high performance liquid chromatography) analysis was examined. Considerable breakdown of chlorophylla was observed after 5 min extraction in boiling ethanol and 2 h extraction in DMSO. Over 50% of the chlorophylla was degraded to chlorophyllidea and there was substantial loss of carotenoids after a 15 h exposure of ground cells to cold 9:1 acetone-water.Mild sonication of ground mat material in 9:1 acetone-water followed by a 4 h extraction at 4 ° C was found to minimise chlorophylla breakdown and dramatically improved the extraction efficiency of chlorophylla, myxoxanthophyll and -carotene. |
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| Keywords: | -carotene chlorophylla cyanobacterial mats HPLC myxoxanthophyll |
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