Lysine catabolism inStreptomyces ambofaciens producer of macrolide antibiotic,spiramycin |
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Authors: | Sophie Untrau Dr Ahmed Lebrihi Pierre Germain Gérard Lefebvre |
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Institution: | (1) Laboratoire de Microbiologie Industrielle et Alimentaire, ENSAIA, Institut National Polytechnique de Lorraine, 2 Avenue de la Forêt de Haye, F-54500 Vandoeuvre, France |
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Abstract: | Spiramycin production was highly stimulated when lysine was used as the sole nitrogen source. This amino acid was catabolized by the -transaminase pathway characterized by dosage of cadaverine aminotransferase (CAT) enzyme. The Kmcadaverine was of 57mM. CAT was highly induced by lysine (634% in comparison with ammonium). Addition of 40mm of ammonium in a culture begun with 20mm of lysine as the sole initial nitrogen source repressed CAT biosynthesis by 24% but did not affect spiramycin production seriously. Addition of 20mm of lysine in a culture started with 40mm ammonium induced CAT biosynthesis of 425%, but did not allow spiramycin production. In these two cases, spiramycin production seems to be conditioned by the nitrogen source initially present in the culture medium. CAT activity was inhibited by ammonium ions (33% at 20mm), whereas lysine had no effects. |
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