| 白介素-10抑制TNF-α诱导的血管平滑肌细胞增殖 |
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| 作者姓名： | OuYang P Peng LS Yang H Wu WY Xu AL |
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| 作者单位： | 中山大学生命科学学院,广州,510275 |
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| 基金项目： | ThisstudywassupportedbyagrantofScienceandTechnologyFoundationofGuangzhou (No JB0 0 0 0 0 44 8165 ) . |
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| 摘 要： | 研究观察了重组人白介素 10 (rhIL 10 )对肿瘤坏死因子 (TNF α)刺激的离体大鼠胸主动脉血管平滑肌细胞增殖、细胞周期及对p4 4 /p4 2丝裂素活化蛋白激酶的影响。实验培养大鼠主动脉血管平滑肌细胞 ,采用MTS/PES法确定血管平滑肌细胞 (vascularsmoothmusclecells,VSMCs)的增殖状态 ;应用流式细胞术测定细胞周期 ;利用p4 4 / 4 2磷酸化抗MAPK抗体的蛋白免疫印迹法测定MAPK蛋白表达。结果显示 :( 1)TNF α处理组与对照组相比 ,TNF α对VSMC增殖具有明显的刺激作用 (P <0 0 5 )。rhIL 10单独应用对VSMCs生长没有影响 (P >0 0 5 )。在TNF α刺激下 ,低至 10ng/ml的rhIL 10可抑制VSMCs的生长 (P <0 0 5 )。流式细胞术测定的结果显示 ,rhIL 10分别可使TNF α作用下的VSMC大部分处于G0 /G1期 ,与对照组相比有明显差异 (P <0 0 1)。 ( 2 )TNF α对p4 4 /p4 2MAPK蛋白表达有显著的增强作用 ,此作用可被rhIL 10抑制。结果提示 ,rhIL 10可抑制TNF α诱导的VSMC增殖及p4 4 /p4 2丝裂素活化蛋白激酶的表达
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| 关 键 词： | 血管平滑肌 白介素－10 丝裂素活化蛋白激酶 细胞垃针 TNF－α 抑制作用 血管硬化 |
| 修稿时间： | 2001年6月17日 |
Recombinant human interleukin-10 inhibits vascular smooth muscle cell proliferation induced by TNF-alpha |
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| OuYang P,Peng LS,Yang H,Wu WY,Xu AL.Recombinant human interleukin-10 inhibits vascular smooth muscle cell proliferation induced by TNF-alpha[J].Acta Physiologica Sinica,2002,54(1):79-82. |
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| Authors: | OuYang Ping Peng Li-Sheng Yang Hong Wu Wen-Yan Xu An-Long |
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| Institution: | College of Life Science, Zhong Shan University, Guangzhou 510275; ls36@zsu.edu.cn |
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| Abstract: | Vessel injury provokes a release in proinflammatory cytokines that influence vascular smooth muscle cell (VSMC) proliferation. The purposes of this study was to determine the effects of recombinant human interleukin-10 (rhIL-10) on rat vascular smooth muscle cell proliferation and the activity of p44/p42 mitogen-activated protein kinase (MAPK) promoted by tumor necrosis factor-alpha (TNF-alpha). Rat aortic VSMCs were cultured and treated with rhIL-10 or TNF-alpha respectively, and then cotreated with rhIL-10 and TNF-alpha. The proliferation of VSMCs was quantified by colormetric assay. Cell cycle analysis was performed by flow cytometry. The p44/42 MAPK activity was evaluated by the immunoblotting technique using anti-p44/42 phospho-MAPK antibody. Compared to control group, TNF-alpha stimulated significantly VSMC proliferation in TNF-alpha group. rhIL-10 alone had no effect on VSMC growth, but significantly inhibited VSMC proliferation induced by TNF-alpha at a dose of 10 ng/ml. The cell number in G(0)/G(1) phase of TNF-alpha and rhIL-10 co-treatment group was higher than that of TNF- alpha group (P<0.01) by flow cytometry analysis. The p44/42 MAPK activity was significantly enhanced by TNF-alpha and the TNF-alpha effect was opposed by rhIL-10. It is suggested that rhIL-10 can inhibit TNF-alpha induced VSMC proliferation and phosphorylation of p44/42 MAPK. |
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| Keywords: | vascular smooth muscle interleukin 10 mitogen activated protein kinase cell proliferation |
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