Functional phosphosite screening for targeted protein-protein interactions by combining phosphoproteomics strategies and mammalian two-hybrid assays |
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| Authors: | Guo Mingquan Xia Zanxian Ma Hong |
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| Institution: | Keck School of Medicine, University of Southern California, 1501 San Pablo St., Los Angeles, CA 90033, USA. mingquag@usc.edu. |
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| Abstract: | An effective new platform for phosphosite mapping and subsequent functional screening was developed to analyze the targeted protein-protein interactions of p300 and CBP with β-catenin. Two novel functional phosphosites, Ser12 of p300 and Ser92 of CBP, were revealed to modulate p300/β-catenin and CBP/β-catenin interactions, respectively. |
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