Regional and developmental variations in metabolite concentration in the rat brain and eye: A study using1H NMR spectroscopy and high performance liquid chromatography |
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| Authors: | C-L Florian Dr S R Williams K K Bhakoo M D Noble |
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| Institution: | (1) The Royal College of Surgeons Unit of Biophysics, Institute of Child Health, 30 Guilford St., WC1N 1EH London, UK;(2) The Ludwig Institute for Cancer Research, 91 Riding House Street, W1P 8BT London, UK;(3) Huntsman Cancer Institute, University of Utah, 84112 Salt Lake City, Utah |
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| Abstract: | Regional and developmental changes in metabolite concentrations were measured by1H NMR spectroscopy and HPLC of perchloric acid extracts from rat brain and eye. The highest concentrations of N-acetylaspartate
were found in grey matter as opposed to white matter with concentration increasing with age from neonate to adult, while the
related compound N-acetylaspartylglutamate was highest in adult optic nerve. Creatine and choline-containing compounds were
present in all regions throughout development, with higher levels of creatine found in grey matter compared to other regions.
Choline-containing compounds were present at the highest concentrations in the eye at all ages examined, and tended to decrease
in concentration to minimum values in adulthood in all regions. The presence of hypotaurine in corpus callosum and optic nerve
was consistent with the metabolic profiles of O-2A progenitor cells and oligodendrocytes, which are cells composing these
tissues. The neurotransmitters glutamate and GABA reached their highest concentrations in the olfactory bulb (higher than
in adult cortex).
Special issue dedicated to Dr. Herman Bachelard. |
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| Keywords: | Rat brain development metabolism 1H NMR brain regions |
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