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Improved Luciferase Tagging System for Listeria monocytogenes Allows Real-Time Monitoring In Vivo and In Vitro |
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| Authors: | Christian U Riedel Ian R Monk Pat G Casey David Morrissey Gerald C O'Sullivan Mark Tangney Colin Hill and Cormac G M Gahan |
| Abstract: | An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies. |
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