IgM rheumatoid factor autoantibody and immunoglobulin-producing precursor cells in the bone marrow of humans |
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| Authors: | S Fong T A Gilbertson R J Hueniken S K Singhal J H Vaughan D A Carson |
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| Affiliation: | 1. Department of Basic and Clinical Research (BCR4), Scripps Clinic and Research Foundation, 10666 North Torrey Pines Road, La Jolla, California 92037 USA;2. Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 4G5, Canada;1. Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA;2. Division of Pulmonary, Critical Care and Sleep Medicine, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, USA;1. Chongqing Key Laboratory for Pharmaceutical Metabolism Research, College of Pharmacy, Chongqing Medical University, 400016, Chongqing, People''s Republic of China;2. Department of Occupational and Environmental Health, School of Public Health, Chongqing Medical University, 400016, Chongqing, People''s Republic of China;3. Molecular Biology Laboratory of Respiratory Disease, Institute of Life Sciences, Chongqing Medical University, 400016, Chongqing, People''s Republic of China;4. Department of Pharmacy, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People''s Republic of China;5. Research Center for Environment and Human Health, School of Public Health, Chongqing Medical University, 400016, Chongqing, People''s Republic of China;1. Key Laboratory of Cardiovascular and Cerebrovascular Medicine, School of Pharmacy, Nanjing Medical University, Nanjing, 211166, China;2. Department of Anesthesiology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, 211166, China;3. Key Laboratory of Targeted Intervention of Cardiovascular Disease, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, Nanjing Medical University, Nanjing, 211166, China |
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| Abstract: | The natures of the IgM rheumatoid factor (RF)-, IgM-, and IgG-secreting cells in the human bone marrow as compared to the peripheral blood, have been investigated by (1) response to the polyclonal B-cell activator, the Epstein-Barr virus (EBV), (2) sensitivity to the S-phase specific antimetabolite hydroxyurea, (3) presence of the BA-1 and Ia antigens on the cell surface, and (4) cell size, as determined by counter flow elutriation. The EBV-inducible bone marrow IgM-RF precursors derived from medium to large B cells that were inhibited by hydroxyurea pretreatment. The marrow total IgM response derived from small to medium size cells, and was only partially inhibited by hydroxyurea. Hydroxyurea had no effect on IgM-RF or IgM synthesis by peripheral blood cells. These results indicate that the marrow EBV-induced IgM-RF response is not representative of the response by peripheral blood cells, moreover; the marrow RF secreting response arises from a dividing cell pool that may represent newly generated autoreactive B cells. |
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