GA3和IAA对慈竹木质素生物合成相关酶活性调控及与木质素含量和S/G的关系

以慈竹为材料，通过叶面喷洒赤霉素（GA₃）和吲哚乙酸（IAA），研究GA₃和IAA对慈竹木质素生物合成相关酶活性调控效应，以及相关酶活性与木质素含量和S/G比值的关系，为慈竹优质栽培和遗传改良提供理论依据。结果表明，GA-350IAA200处理对4CL酶活性具有促进作用；而GA-3200IAA50在处理后50天时对4CL酶活性起到明显的抑制作用，而且改变了4CL酶活性的动态变化趋势。GA₃和IAA对慈竹CAD酶活性具有促进作用；对PAL酶活性具有明显的抑制作用。相关分析结果表明，经过GA₃和IAA处理后，4CL酶活性与木质素含量和S/G比值呈不显著的负相关关系；PAL酶活性与木质素含量呈不显著的正相关关系；CAD酶活性与木质素含量和S/G比值呈不显著的正相关或负相关关系。结论，GA₃和IAA对慈竹木质素生物合成相关酶活性具有调控效应，而且这种调控作用与GA₃和IAA的浓度和配比有关。

Effects of GA3 and IAA Spraying on the Correlated Enzyme Activities in the Lignin Biosynthesis of Neosinocalamus affinis and its Relationships with the Lignin Content and S/G

The effects of spraying the different concentration and combination of gibberellin acid (GA₃) with indol-3-yl acetic acid (IAA) on the correlated enzyme activities in the lignin biosynthesis of Neosinocalamus affinis and their relationships with the lignin content and S/G were studied. It aims to provide the theoretical evidence for the quality cultivation and genetic improvement. The results showed that the treatment of GA-350IAA200 increased the enzyme activities of 4CL, but the GA-3200IAA50 repressed the enzyme activities of 4CL about 50 days after spraying GA₃ and IAA, and changed the tends of dynamic changes of 4CL enzyme activities compared with the control. The GA₃ and IAA could improve the CAD enzyme activities and significantly repress the PAL enzyme activities. After spraying the different concentration and combination of GA₃ with IAA, the correlated analysis results showed that the negative relationships of the 4CL enzyme activities with the lignin content and S/G value were found, the positive relationships of the PAL enzyme activities with the lignin content was not significant, the positive or negative relationships of the CAD enzyme activities with the lignin content and S/G value were not significant. The correlated enzyme activities in the lignin biosynthesis were regulated by spraying the different concentration and combination of GA₃ with IAA, but the effects of GA₃ and IAA on the correlated enzyme activities were related to their concentration and combination.