ICR小鼠肠道中乳酸菌组成的克隆文库研究

目的对健康雄性ICR小鼠肠道内乳酸菌的组成结构进行研究。方法收集10只健康雄性ICR小鼠的新鲜粪便样品,提取粪便样品中微生物的总DNA,采用乳酸菌类群特异性引物（Lac1）和细菌通用性引物（1391r）的组合扩增16SrRNA基因并构建乳酸菌特异性克隆文库,研究小鼠肠道内各种乳酸菌的组成和比例。结果克隆文库的分析结果表明罗伊乳杆菌（Lactobacillus reuteri）和约氏乳杆菌（Lactobacillus johnsonii）为ICR小鼠肠道内的优势种,其他还包括鼠乳杆菌（Lactobacillus murinus）、阴道乳杆菌（Lactobacillus vaginalis）、肠乳杆菌（Lacto-bacillus intestinalis）等乳酸菌以及一个潜在的乳酸菌新种。结论健康ICR小鼠肠道内乳酸菌的多样性较高;L.re-uteri种可能具有较高的菌株水平多样性。

Clone library analysis of Lactic acid bacteria composition in ICR mice

Objective To study the composition of Lactic acid bacteria in the intestine of healthy ICR mice. Methods Fresh fecal samples from 10 healthy male ICR mice were collected and the total genomic DNA was extracted from each sample. Lactic acid bacteria （LAB） group specific primer （ Lacl ） and universal primer for bacteria （1391r） were used to amplify 16S rRNA gene and construct LAB group specific clone library to analyze the species composition of LAB. Results The results of clone library showed that Lactobacillus reuteri and Lactobacillus johmonii were the most predominant species in gut of mice. Lactobacillus murinus,Lactobacillus vaginalis,Lactobacillus intestlnalis and a potentially new species were also detected. Conclusion The composition and structure of Lactobacillus in healthy ICR mice have high diversity and the species of Lactobacillus reuteri may have high microdiversity at strain level.