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Increase of transferrin receptors in regenerating rat liver cells after partial hepatectomy |
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| Authors: | I Tei Y Makino T Kadofuku I Kanamaru K Konno |
| Institution: | 1. Department of Biochemistry, School of Medicine, Showa University 1-5-8, Hatanodai, Shinagawa-ku, Tokyo 142, Japan;2. Central Chemical Laboratory, School of Medicine, Showa University 1-5-8, Hatanodai, Shinagawa-ku, Tokyo 142, Japan |
| Abstract: | The change of transferrin receptors in regenerating rat liver cells after partial hepatectomy was demonstrated. The binding of 125I-labeled transferrin to the cells began to increase after 24 h of partial hepatectomy and reached about double that of the non-operated normal rat liver cells. A Scatchard analysis of binding parameters showed 1.62 X 10(5) (Ka: 1.04 X 10(7) M-1) in normal cells and 3.36 X 10(5) (Ka: 1.23 X 10(7) M-1) in regenerating cells. This reflected on increase of transferrin receptor number and little change occurred in the binding affinity between transferrin and its surface receptor. |
| Keywords: | MEM minimum essential medium PBS phosphate buffered saline without calcium or magnesium |
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