Identification and characterization of two internal cleavage and polyadenylation sites of parvovirus B19 RNA |
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Authors: | Yoto Yuko Qiu Jianming Pintel David J |
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Institution: | Department of Molecular Microbiology and Immunology, 471f Life Sciences Center, University of Missouri-Columbia, 1201 E. Rollins Rd., Columbia, MO 65211-7310, USA. |
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Abstract: | Polyadenylation of B19 pre-mRNAs at the major internal site, (pA)p1, is programmed by the nonconsensus core cleavage and polyadenylation specificity factor-binding hexanucleotide AUUAAA. Efficient use of this element requires both downstream and upstream cis-acting elements and is further influenced by an adjacent AAUAAC motif. The primary hexanucleotide element must be nonconsensus to allow efficient readthrough of P6-generated pre-mRNAs into the capsid-coding region. An additional cleavage and polyadenylation site, (pA)p2, 296 nucleotides downstream of (pA)p1 was shown to be used following both B19 infection and transfection of a genomic clone. RNAs polyadenylated at (pA)p2 comprise approximately 10% of B19 RNAs that are polyadenylated internally. |
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