In vivo mutant frequency rises among breast cancer patients after exposure to high doses of gamma-radiation |
| |
Authors: | K Messing W E Bradley |
| |
Institution: | 1. Départment des Sciences Biologiques, Université du Québec à Montréal, Montreal, Que. H3C 3P8, Canada;2. Institut du Cancer de Montréal, Hôpital Notre-Dame, Montreal, Que. H2L 4M1 Canada |
| |
Abstract: | Human in vivo mutant frequencies can be measured by cloning freshly isolated lymphocytes in selective media containing 6-thioguanine (TG). This method was applied to monitoring environmental mutagenesis, by studying lymphocytes separated from peripheral blood of 12 cancer patients undergoing radiotherapy. Before therapy, cancer patients had an average 8.6 X 10(-6) mutants/cell, compared to 2.4 X 10(-6) mutants/cell for heart patients and 1.1 X 10(-6) mutants/cell for healthy controls. After exposure of cancer patients to 50 Gy of gamma-radiation delivered to the treated area, or an estimated 4 Gy received by each lymphocyte, patients averaged 36.8 X 10(-6) mutants/viable cell. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|