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Determination of the maximum and minimum lethal temperatures (LT50) for Loxosceles intermedia Mello-Leitão, 1934 and L. laeta (Nicolet, 1849) (Araneae, Sicariidae)
Authors:Marta L Fischer  Joo Vasconcellos-Neto
Institution:

a Departamento de Biologia, Centro de Ciências Biológicas e da Saúde, Pontifícia Universidade Católica do Paraná, Av. Silva Jardim 1664/1101. CEP, Curitiba 80250-200, Brazil

b Núcleo de Estudos do Comportamento Animal-Grupo de Pesquisa Biologia ambiental, CNPq/PUCPR, Brazil

c Departamento de Zoologia, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), C.P. 6109, Campinas, S.P. CEP 13083-970, Brazil

Abstract:In this study, the maximum and minimum lethal temperatures (LT50) of L. intermedia and L. laeta were determined in two treatments: gradual heating (25–50°C) and cooling (25°C to ?5°C), and 1 h at a constant temperature. In gradual temperatures change, L. intermedia mortality started at 40°C and the LT50 was 42°C; for L. laeta, mortality began at 35°C and the LT50 was 40°C. At low temperatures, mortality was registered only at ?5°C for both species. In the constant temperature L. intermedia showed a maximum LT50 at 35°C and L. laeta at 32°C; the minimum LT for both species was ?7°C.
Keywords:Lethal temperature  Thermal tolerance  Loxosceles  Behavior  Laboratory experiments
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