L-Lysine production by mutants of Bacillus licheniformis |
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Authors: | Hiroshi Hagino Setsuki Kobayashi Kazumi Araki |
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Affiliation: | (1) Kiyoshi Nakayama Tokyo Research Laboratory, Kyowa Hakko Kogyo Co. Ltd., 3-6-6, Asahi-machi, Machida-shi, Tokyo, Japan |
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Abstract: | Summary A bacterium able to grow at 46°C was isolated from soil and identified asBacillus licheniformis. L-Lysine producing mutants were derived from the bacterium by the introduction of resistance to S-(2-amino)-ethylcysteine (thialysine) and auxotrophy.One of the mutants, HBR-2 (Thialysiner, Leu–, Homoserine–), produced L-lysine at a concentration of 30 mg/ml in a molasses medium containing 10% reducing sugar. |
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