Enhancement of Apoptosis in Developing Chick Neural Retina Cells by Basic Fibroblast Growth Factor |
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| Authors: | Yoshiko Yokoyama,Satoshi Ozawa,Yousuke Seyama,Hideo Namiki,&dagger Yokichi Hayashi,&Dagger Kazuhiko Kaji,§ Kazuhiko Shirama,&# Masaki Shioda, Kazutaka Kano |
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| Affiliation: | Advanced Research Center for Science and Engineering, Waseda University, Tokyo;; Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Tokyo;; Department of Anatomy, Tokyo Medical College, Tokyo;; Department of Molecular and Cell Biology, Nagano College of Nursing, Nagano;; Laboratory of Cell and Molecular Biology of Aging, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka;and; Department of Biological Science, Faculty of Science, Kumamoto University, Kumamoto, Japan |
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| Abstract: | Abstract: To evaluate the role of various growth factors in naturally occurring cell death during development of the neural retina, we examined the effects of such factors on the nuclear morphology and the size of DNA in cultured chick embryonic neural retina cells. Basic fibroblast growth factor (bFGF) increased internucleosomal cleavage of DNA and nuclear fragmentation in a time- and dose-dependent manner. The effect was inhibited by anti-bFGF antibody, suramin, and cycloheximide. Epidermal growth factor, platelet-derived growth factor, nerve growth factor, tumor necrosis factor-α, and dexamethasone had no effect. These results provide evidence that bFGF may eventually act as a lethal factor inducing apoptotic cell death during the development of the neural retina in chick embryo. |
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| Keywords: | Basic fibroblast growth factor Apoptosis Neural retina DNA cleavage Nuclear fragmentation Suramin |
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