云南油杉原胚发育过程中组织化学的变化

应用组织化学方法对云南油杉(Keteleeria evelyniana Mast)受精前后,原胚及幼胚形成早期细胞内的DNA、RNA、碱性蛋白质,酸性蛋白质及多糖物质进行了观察。结果表明,DNA在卵核受精前后为孚尔根弱正反应。在原胚及早期幼胚发育过程中,胚原细胞核DNA含量恢复正常,RNA及酸性蛋白质含量均较丰富,特别是在胚原细胞内。新细胞质中碱性蛋白质呈负反应,而DNA、RNA、酸性蛋白质及多糖物质均呈现正反应。

CHANGES ON THE HISTOCHEMICAL LOCALIZATION IN THE DEVELOPMENT PROCESS OF PROEMBRYO OF KETELEERIA EVELYNANA MAST

A primary observation on the DNA, RNA, basic protein, acid protein and polysaccharide at the process of fertilization in the proembryo and early young embryo of Keteleeria evelyniana Mast has been made through histochemical methods. The following results are obtained:The DNA staining reaction was weaker by using Feulgen reation before and after fertilization within the egg nucleus. After formed proembryo and early young embryo, the content of DNA in the nuclei of the initials became normal. The RNA and acid protein contents are rich during the whole development, especially within the embryo initials. However, A-S staining of the basic protein was negative within the neocytoplasm, but the DNA, RNA, acid protein and polysaccharide were positive.