Cloning and characterisation of a novel cry1H gene effective against lepidopterous larvae from a Bacillus thuringiensis strain |
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| Authors: | Zonglan Yu Liye He Li Gong Ganghui Huang Yao Liu Ping Li |
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| Institution: | 1. Rice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, P.R. China;2. Key Laboratory of Southwest Crop Gene Resource &3. Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an, Sichuan 625014, P.R. China |
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| Abstract: | We characterised a novel holotype cry gene (cry1Hc1) harboured in BN23-5 Bt strain which was isolated from soil samples in Sichuan, China. In this study, the full length of the cry1Hc1 gene was cloned from the strain. The cry1Hc1 gene was inserted into a shuttle vector (pSTK) and expressed in an acrystalliferous mutant Bacillus thuringiensis HD73?. In this transformant, cry1Hc1 was expressed and diamond-shaped parasporal crystals were formed. The resulting insecticidal activity showed that the Cry1Hc1 protein exhibited high larvicidal activity against larvae of Plutella xyllostella and Ostrinia furnacalis with lethal concentration 50 of 55.21 and 118.44?μg/ml, respectively. Sequence analysis of the gene was also performed; the Cry1Hc1 protein retained eight conserved regions commonly found in the existing Cry proteins. |
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| Keywords: | Bacillus thuringiensis cry1Hc1 gene Lepidopterous pests novel gene parasporal crystal |
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