Nematicidal action of chitinases produced by the fungus Monacrosporium thaumasium under laboratorial conditions |
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| Authors: | Filippe Elias de Freitas Soares José Humberto de Queiroz Jackson Victor de Araújo Paula Viana Queiroz Angélica de Souza Gouveia Emy Hiura |
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| Institution: | 1. Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Vi?osa, Vi?osa-MG, Brazilfilippeufv@yahoo.com.br;3. Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Vi?osa, Vi?osa-MG, Brazil;4. Departamento de Veterinária, Universidade Federal de Vi?osa, Vi?osa-MG, Brazil;5. Departamento de Veterinária, Universidade Vila-Velha, Vila Velha, Espirito Santo, Brazil |
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| Abstract: | Nematophagous fungi produce chitinases that may be important in the process of infection of eggs and larvae of nematodes. This study aimed to produce, purify, characterise and test the nematicidal action of extracellular chitinases produced by Monacrosporium thaumasium on Panagrellus redivivus. Mycelia from M. thaumasium were used to inoculate a solid medium for chitinase production. The enzymes were purified using a specific technique of adsorption for chitinases. The chitinase activity was determined at different pHs and temperatures. NF34 produced two distinct chitinases (27 and 30 kDa). After 72 hours, these enzymes provided a significant reduction (80%; p < 0.01) of the number of P. redivivus larvae, compared to control. It was shown that isolate NF34 produced chitinases with nematicidal activity. Thus, other experimental designs on geohelminths or even arthropods that transmit diseases may become a new aspect of the field of study of biological control using predatory nematophagous fungi. |
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| Keywords: | nematophagous fungi Monacrosporium thaumasium chitinase |
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