Fluorescence quenching method for the determination of bleomycins A5 and A2 with halofluorescein dyes |
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| Authors: | Jiangtao Liu Zhongfang Liu Xiaoli Hu Ling Kong Shaopu Liu |
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| Affiliation: | 1. School of Chemistry and Chemical Engineering, Southwest University, Beibei, People's Republic of China;2. Department of Chemistry and Environment Engineering, Fuling Normal University, People's Republic of China |
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| Abstract: | In weak acidic medium, the anticancer antibiotics bleomycin A5 (BLMA5) and bleomycin A2 (BLMA2) bind with halofluorescein dyes, such as erythrosin (Ery), eosin Y (EY) and eosin B (EB), to form ion‐association complexes, which causes fluorescence quenching of halofluorescein dyes. The quenching values (ΔF) are directly in proportional to the concentrations of bleomycins over the range 0.09–2.5 µg/mL. Based on this, a fluorescence quenching method for the determination of BLMA5 and BLMA2 has been developed. The dynamic range is 0.12–2.5 µg/mL for the determination of BLMA5 and 0.09–2.0 µg/mL for BLMA2, with detection limits (3σ) of 0.04 µg/mL for BLMA5, 0.03 µg/mL for BLMA2, respectively. It has been applied to determine the two antibiotics in human serum, urine and rabbit serum samples. The recovery is in the range 90–102%. In this work, the optimum reaction conditions and the spectral characteristics of the fluorescence are investigated. The reasons for fluorescence quenching are discussed, based on the fluorescence theory. Copyright © 2007 John Wiley & Sons, Ltd. |
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| Keywords: | bleomycin A5 bleomycin A2 halofluorescein dyes fluorescence quenching |
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