Determination of leukotriene E4 in human urine using liquid chromatography–tandem mass spectrometry |
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Authors: | Michinao Mizugaki Takanori Hishinuma Naoto Suzuki |
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Abstract: | A liquid chromatographic–tandem mass spectrometric (LC–MS–MS) method was developed for the quantitation of urinary leukotriene E4 (LTE4). LTE4 and its internal standard were extracted by solid-phase extraction and analysed using LC–MS–MS in the selected reaction monitoring (SRM) mode. A good linear response over the range of 10 pg to 10 ng was demonstrated. The accuracy of added LTE4 ranged from 97.0% to 108.0% with a mean and SD of 100.6±2.4%. We detected LTE4 (63.1±18.7 pg/mg creatinine, n=10) in healthy human urine. This method can be used to determine LTE4 in biological samples. |
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Keywords: | Leukotrienes |
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