The use of spectral fluorescence methods to detect changes in the phytoplankton community |
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Authors: | Seppälä Jukka Balode Maija |
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Institution: | 1.Tvärminne Zoological Station, University of Helsinki, FIN-10900, Hanko, Finland ;2.Institute of Aquatic Ecology, University of Latvia, Miera 3, LV-2169, Salaspils, Latvia ; |
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Abstract: | In vivo fluorescence methods are efficient toolsfor studying the seasonal and spatial dynamics ofphytoplankton. Traditionally
the measurements are madeusing single excitation-emission wavelengthcombination. During a cruise in the Gulf of Riga(Baltic
Sea) we supplemented this technique bymeasuring the spectral fluorescence signal (SFS) andfixed wavelength fluorescence intensities
at theexcitation maxima of main accessory pigments. Thesemethods allowed the rapid collection of quantitativefluorescence
data and chemotaxonomic diagnostics ofthe phytoplankton community. The chlorophylla-specific fluorescence intensities (R)
and thespectral fluorescence fingerprints were analysedtogether with concentrations of chlorophyll a indifferent algal size-groups,
phytoplankton biomass andtaxonomic position. The lower level of R in thesouthern gulf was related to the higher proportion
ofcyanobacteria relative to total biomass and the lowerabundance of small algae. The phycoerythrinfluorescence signal was
obviously due to the largecyanobacteria. The basin-wide shift in the shape ofchlorophyll a excitation spectra was caused bythe
variable proportions of differently pigmentedcyanobacteria, diatoms and cryptomonads.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | in vivofluorescence spectrofluorometry phytoplanktonpigments phytoplankton community structure Gulf of Riga |
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