Identification of Brassica oleracea monosomic alien chromosome addition lines with molecular markers reveals extensive gene duplication |
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Authors: | J. Mitchell McGrath Carlos F. Quiros John J. Harada Benoit S. Landry |
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Affiliation: | (1) Department of Vegetable Crops, University of California, 95616 Davis, CA, USA;(2) Department of Botany, University of California, 95616 Davis, CA, USA;(3) Agriculture Canada Research Center, P.O. Box 457, J3B 6Z8 St-Jean, Quebec, Canada;(4) Present address: Department of Biology, University of Michigan, 48109-1048 Ann Arbor, MI, USA |
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Abstract: | Summary Chromosomes of Brassica oleracea (2n=18) were dissected from the resynthesized amphidiploid B. napus Hakuran by repeated backcrosses to B. campestris (2n=20), creating a series of monosomic alien chromosome addition line plants (2n=21). Using morphological, isozyme and restriction fragment length polymorphism markers (RFLPs), 81 putative loci were identified. Of nine possible synteny groups, seven were represented in the 25 monosomic addition plants tested. Sequences homologous to 26% of the 61 DNA clones utilized (80% were cDNA clones) were found on more than one synteny group, indicating a high level of gene duplication. Anomalous synteny associations were detected in four 2n=21 plants. One of these plants showed two markers from one B. oleracea chromosome associated with a second complete B. oleracea synteny group, suggesting translocation or recombination between non-homologous chromosomes in Hakuran or the backcross derivatives. The other three 2n=21 plants each contained two or more B. oleracea synteny groups, suggesting chromosome substitution. |
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Keywords: | Restriction fragment length polymorphism Chromosome substitution Non-homologous recombination Genetic stocks Aneuploidy |
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