两个玉米矮花叶病显性互补抗病基因的发现和定位

玉米矮花叶病是世界普通发生危害严重的玉米病毒病害之一，迄今为止，只有少数几个抗病基因被发现并定位，优良自交系四一是鉴定出定的玉米筹花叶病新抗源，它表现为全生育抗性，通过连续两年的经典遗传学研究发现，四一的成株期抗性表现为一种新的抗病遗传模式，该抗性是由两个显性互补抗病基因控制，87对微卫星标记分析进一步证实了以上推论，并把两个抗病基因分别定位在第三和第六染色体上，第三染色体上的抗病基因与微卫星标记phi029相距14.5cM，第六染色体上的抗病基因与微卫星标记phil26相距7.2cM.

Identification and Molecular Tagging of Two Complementary Dominant Resistance Genes to Maize Dwarf Mosaic Virus

Maize dwarf mosaic is one of the devastating and widespread viral diseases in the world. So far, only a few genes were identified and mapped in the resistant materials. A new resistant elite inbred line Siyi was identified with resistance to maize dwarf mosaic virus strain B at early and adult stage. Two complementary dominant genes conditioned the resistance, with a new genetic model, of the maize inbred line were found at adult stage by the genetic analysis based on parents, F 1, F 2 and backcrosses in two years. The microsatellite analysis of a F 2 population from the cross between Siyi and Mo17 was used to identify the two resistance genes on chromosome 3 and 6 respectively by 87 pairs of microsatellite markers. The linkage distance between phi029 and the one resistance gene on chromosome 3 is 14.5cM, and phi126 to the other on chromosome 6 is 7.2cM.