| 蓝猪耳合子和二胞原胚细胞的分离(简报) |
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| 引用本文： | 杨延红,王雅英,叶祖云,田惠桥. 蓝猪耳合子和二胞原胚细胞的分离(简报)[J]. 分子细胞生物学报, 2008, 41(1): 75-80 |
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| 作者姓名： | 杨延红 王雅英 叶祖云 田惠桥 |
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| 作者单位： | 厦门大学生命科学学院,厦门261005 |
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| 摘 要： | 合子是被子植物个体发育的第一个细胞.合子分裂的规律性是植物早期胚胎发生的种属甚至是科的特征,也是控制早期个体发育的关键阶段.
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| 关 键 词： | 合子 二胞原胚 分离 蓝猪耳 蓝猪耳 合子 胚细胞 分离 简报 CELLS 早期个体发育 控制 特征 种属 胚胎发生 被子植物 规律 |
| 收稿时间： | 2007-09-19 |
| 修稿时间： | 2007-12-05 |
ISOLATION OF ZYGOTES AND APICAL AND BASAL CELLS OF BICELLUARE PROEMBRYO FROM TORENIA FUORNIERI |
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| YANG Yan Hong,WANG Ya Ying,YE Zu Yun,TIAN Hui Qiao. ISOLATION OF ZYGOTES AND APICAL AND BASAL CELLS OF BICELLUARE PROEMBRYO FROM TORENIA FUORNIERI[J]. Journal of Molecular Cell Biology, 2008, 41(1): 75-80 |
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| Authors: | YANG Yan Hong WANG Ya Ying YE Zu Yun TIAN Hui Qiao |
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| Affiliation: | School of Life Sciences, Xiamen University, Xiamen 361005. |
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| Abstract: | Torenia fournieri is a special plant with embryo sac partly protruding through the micropyle of ovule, and the cells of egg apparatus can be clearly observed using light microscope. Zygotes and the cells of bicellular proembryo could be isolated using enzymatic digestion. In the solution containing 0.05?llulase and 0.05% pectinase, 14-15 zygotes were isolated from 50 ovules in lh. In the solution containing 0.2% cellulose, 0.4% hemicellulase and 0.2% pectinase, 19 pairs of apical and basal cells of bicellular proembryo could be isolated from 50 ovules in 1 h. The isolated zygotes and epical and basal cells were collected using a micromanipulator up to a number which will prepare for suing molecular methods to probe the mechanism of early embryogenesis of higher plants. |
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| Keywords: | Bicellular proembryo. Isolation. Torenia fournieri. Zygote |
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