The use of pulsed field electrophoresis using diversely directed electrical fields for establishing the degree of similarity of three Pseudomonas cultures]

It has been shown that from the group of three restriction endonucleases (XbaI, Uba 78I, DraI) only XbaI digests the native Pseudomonas genomic DNA into the number of fragments, sufficient for their comparative analysis. Optimal electrophoresis conditions were adjusted for the better DNA restricts separation. Each Pseudomonas culture gives unique patterns of DNA restriction. The calculation of the conservative fragments fraction content in the samples led us to the conclusion that P. fluorescens differs equally from both P. fluorescens 80 and P. alcaligenes. The P. fluorescens 80 DNA contains a relatively larger number of fragments equal in size with those of P. alcaligenes DNA, than DNA of P. fluorescens. We have concluded that the systematics of the Pseudomonas genus is still imperfect and that the analysis of DNA restriction patterns is necessary for the estimation of the similarity level of cultures studied.