Radiosensitization by diospyrin diethylether in MCF-7 breast carcinoma cell line |
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Authors: | Binod Kumar Jayashree Joshi Amit Kumar Badri N Pandey Banasri Hazra Kaushala P Mishra |
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Institution: | (1) Department of Pharmaceutical Technology, Jadavpur University, Raja S.C. Mullick Road, Jadavpur, Calcutta, West Bengal, 700032, India;(2) Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India |
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Abstract: | The development of radio-resistant tumor cells might be overcome by the use of tumor selective cytotoxic agents in combination
with radiation treatment of cancer. Thus, we are exploring the radiomodifying potential of D7, a tumor-inhibitory compound
derived from a plant product, diospyrin, in breast carcinoma cells, MCF-7. The present study indicated that D7 could enhance
the radiation-induced cytotoxicity and apoptosis through down-regulation of the anti-apoptotic Bcl-2 and COX-2 gene expression,
and up-regulation of pro-apoptotic genes, like p53 and p21. The higher expression of PUMA, a pro-apoptotic protein was also
observed in the combination treatment. Effect of D7 on up-regulation of p21 expression in irradiated MCF-7 cells was concomitant
with the cell cycle arrest in the G1 phase. Thus, it was concluded that D7 could sensitize the effect of radiation in breast
carcinoma by regulating the gene expression involved in cell cycle and apoptosis. |
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Keywords: | Quinonoids Diospyrin Radiation Apoptosis p53 Bcl-2 |
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