The effect of 17O on the relaxation of an amide proton within a hydrogen bond |
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Authors: | Christopher J Halkides Alfred G Redfield |
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Institution: | (1) Department of Biochemistry, Brandeis University, 415 South Street, 02254 Waltham, MA, USA |
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Abstract: | Summary The relaxation rates of the multiple-quantum coherence for the amide hydrogen of Gly13 in ras p21·GDP were determined in the presence and absence of 17O labeling in the -phosphate of GDP. No significant difference could be observed between labeled and unlabeled samples, in spite of the fact that the hydrogen bond from the amide group of Gly13 to the -phosphate is shorter than is typical, based on its chemical shift. For macromolecules in which an oxygen atom is the acceptor of a hydrogen bond, dipolar coupling between 17O and hydrogen is unlikely to be observable, except for extremely short H-bonds.Abbreviations p21
21-kD protein product of the human H-ras gene
- GMPPCP
guanylyl , -methylene]diphosphate
- HPLC
high-performance liquid chromatography
- CSA
chemical shift anisotropy
- HMQC
heteronuclear multiple-quantum coherence |
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Keywords: | Dipolar relaxation 17O Hydrogen bond Amide p21 Ras |
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