Electron microscopy of G-banded human mitotic chromosomes |
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Authors: | Xin Xu Madeline Wu |
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Institution: | (1) Department of Biological Sciences, University of Maryland Baltimore County, 21228 Catonsville, MD, USA;(2) Present address: Department of Biology, East China Normal University, Shanghai, The People's Republic of China |
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Abstract: | Trypsin-treated human metaphase chromosomes stained with Giemsa and uranyl acetate showed clear, reproducible band structures under the transmission electron microscope (TEM). The banding pattern observed with TEM corresponded very closely to the G-band pattern visualized by light microscopy. The TEM images were used for karyotype analyses. Trypsin-treated chromosomes stained with uranyl acetate alone also showed clear G-bands under TEM. Shadow casting in addition to uranyl acetate staining revealed more structural detail of the chromosomes. Chromosome fibers, 200 Å–300 Å in diameter, were observed in the interband regions. Most chromosomes showed the major G-bands under the higher TEM magnification wit0out any trypsin treatment. |
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