Baculovirus-Mediated Gene Expression in Zebrafish |
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Authors: | Mahendra Wagle Suresh Jesuthasan |
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Institution: | (1) , Fish Developmental Genetics Laboratory, IMA, 1 Research Link, 117604, Singapore, |
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Abstract: | Abstract
In an effort to misexpress genes in zebrafish, we tested the ability of baculovirus to infect and drive gene expression in
embryos. By injecting virus into specific tissues and using appropriate promoters, both the location and time of gene expression
could be controlled. Using a virus with 2 different promoters, LacZ and GFP could be expressed independently. The efficiency
of expression appears to depend on the promoter used. As a test of this system, baculovirus was used to ectopically express
ephrinB2a in the presomitic mesoderm. EphrinB2a is normally expressed in the posterior region of developing somites, and baculovirus-mediated misexpression caused abnormal
somite boundary formation. Baculovirus can thus be used as a tool for gene misexpression experiments in the zebrafish, especially
when localized misexpression is required late in development. |
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Keywords: | baculovirus gene expression zebrafish |
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