| 猪繁殖与呼吸综合症病毒GP5蛋白原核表达初步研究 |
| |
| 引用本文： | 陈勇军,苏鑫铭,高晓飞,郑其升,于春梅,曹瑞兵,周斌,陈溥言.猪繁殖与呼吸综合症病毒GP5蛋白原核表达初步研究[J].Virologica Sinica,2005,20(4):441-443. |
| |
| 作者姓名： | 陈勇军 苏鑫铭 高晓飞 郑其升 于春梅 曹瑞兵 周斌 陈溥言 |
| |
| 作者单位： | 南京农业大学农业部动物疫病诊断与免疫重点开放实验室 江苏南京210095
(陈勇军，苏鑫铭，高晓飞，郑其升，于春梅，曹瑞兵，周斌)，南京农业大学农业部动物疫病诊断与免疫重点开放实验室 江苏南京210095(陈溥言) |
| |
| 基金项目： | 国家“863”高技术发展计划资助项目(2001AA249012) |
| |
| 摘 要： | 猪繁殖与呼吸综合症是由猪繁殖与呼吸综合症病毒(Porcine reprodutive and respiratory syn-drome virus,PRRSV)引发的病毒性传染病,病症主要表现为怀孕母猪早产、流产、木乃伊胎以及仔猪呼吸症状和高死亡率1,2]。该病于1987年首先在美国爆发3],1990年德国亦发现此病,并迅速传播到欧洲其他国家。我国在1996年报道有该病流行4],由于该病的迅速蔓延使整个世界养猪业遭受了巨大的经济损失。PRRSV属于动脉炎病毒科(Arteriviridae)成员,为有囊膜的单股正链RNA病毒,基因组为15Kb,含有7个开放阅读框, ORF ( Open readingframes,ORF),…
|
| 关 键 词： | 猪繁殖与呼吸综合症病毒 GP5蛋白 原核表达 |
| 收稿时间： | 2005-01-04 |
Preliminary Studies of Prokaryotic Expression of GP5 of Porcine Reproductive and Respiratory Syndrome Virus |
| |
| Chen YongJun;Su XinMing;Gao XiaoFei;Zheng JiSheng;Yu ChunMei;Cao RuiBing;Zhou Bin;Chen PuYan.Preliminary Studies of Prokaryotic Expression of GP5 of Porcine Reproductive and Respiratory Syndrome Virus[J].中国病毒学(英文版),2005,20(4):441-443. |
| |
| Authors: | Chen YongJun;Su XinMing;Gao XiaoFei;Zheng JiSheng;Yu ChunMei;Cao RuiBing;Zhou Bin;Chen PuYan |
| |
| Abstract: | The envelope glycoprotein (GP5) is one of the major structural proteins of Porcine reproductive and respiratory syndrome virus (PRRSV). In this report, the N-terminal hydrophobic sequence of envelope glycoprotein gene (orf5) was deleted by PCR. The gene was subcloned into prokaryotic expressing vector pET-28 a( ) , the recombinant plasmid named pET-GP5 was transformed into E.coli cell BL21. SDS-PAGE and Western-blot indicated that the orf5 gene was expressed successfully and the recombinant fusion protein, which was about 20.8kDa, had immunologically reactive activity. The studies lay foundations for further study on the development of vaccines for PRRSV. |
| |
| Keywords: | Porcine reproductive and respiratory syndrome virus GP5 protein Prokaryotic expression |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
