锂对小鼠高增殖潜能集落形成细胞和粒巨噬系祖细胞体外增殖的影响

本文观察了锂对ＢＡＬＢ／Ｃ小鼠骨髓高增殖潜能集落形成细胞（ＨＰＰ－ＣＦＣ）和粒巨噬系祖细胞ＣＦＵ－ＧＭ体外增殖的影响。ＨＰＰ－ＣＦＣ集落由ＩＬ－１、ＩＬ－６、ＷＥＨＩ３条件培养液（ＷＥＨＩ３－ＣＭ，含有ＩＬ－３）及Ｌ９２９条件培养液（Ｌ９２９－ＣＭ，含有Ｍ－ＣＳＦ）所支持，而ＣＦＵ－ＧＭ由ＷＥＨＩ３－ＣＭ所支持。结果显示，ＬｉＣｌ浓度在０．４－２ｍｍｏｌ／Ｌ时呈现剂量依赖性抑制ＨＰＰ－ＣＦＣ增殖；而在０．４－１ｍｍｏｌ／Ｌ的浓度范围内，则对ＣＦＵ－ＧＭ的增殖起剂量依赖性促进作用。这些结果提示ＬｉＣｌ对ＨＰＰ－ＣＦＣ和ＣＦＵ－ＧＭ的作用不同，可能锂有诱导ＨＰＰ－ＣＦＣ向成熟细胞分化的作用

EFFECTS OF LITHIUM ON THE PROLIFERATION OF MURINE HIGH PROLIFERATIVE POTENTIAL COLONY FORMING CELLS AND GRANULOCYTE MACROPHAGE COLONY FORMING UNIT IN VITRO

The effects of LiCl on the proliferation of high proliferative potential colony forming cells (HPP CFC) and granulocyte macrophage colony forming unit (CFU GM) from bone marrow of BALB/c mice were observed. The colonies of HPP CFC were supported by IL 1,IL 6, WEHI 3 conditioned medium (WEHI 3 CM, which contained IL 3) and L929 conditioned medium (L929 CM,which contained M CSF); the colonies of CFU GM were supported by WEHI 3 CM. It was shown that LiCl at concentrations ranging from 0 4mmol/L to 2mmol/L significantly inhibited the proliferation of HPP CFC ( P <0 01), but concentrations ranging from 0 4 mmol/L to 1mmol/L significantly increased the production of CFU GM ( P <0 05),both effects displayed in dose dependent manners. The different effects of LiCl on the proliferation of HPP CFC and CFU GM suggest that LiCl may induce HPP CFC differentiation into more mature cells.