Identification and genetic effect of a variable duplication in the promoter region of the cattle ADIPOQ gene |
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| Authors: | L. Zhang M. Yang C. Li Y. Xu J. Sun C. Lei X. Lan C. Zhang H. Chen |
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| Affiliation: | 1. College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, , Yangling, Shaanxi, 712100 China;2. United States Department of Agriculture‐Agricultural Research Service, Bovine Functional Genomics Laboratory, , Beltsville, MD, 20705 USA;3. Institute of Cellular and Molecular Biology, Jiangsu Normal University, , Xuzhou, Jiangsu, 221116 China |
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| Abstract: | In cattle, the ADIPOQ gene is located in the vicinity of the quantitative trait locus (QTL) affecting marbling, the ribeye muscle area and fat thickness on BTA1. In our study, a novel variable duplication (NW_003103812.1:g.9232067_9232133dup) in the bovine ADIPOQ promoter region was identified and genotyped in seven Chinese cattle breeds. Using a reporter assay, we demonstrated that g.9232067_9232133dup decreased the basal transcriptional activity of the ADIPOQ gene in the 3T3‐L1 and C2C12 cells. Furthermore, g.9232067_9232133dup suppressed the mRNA expression of the gene in adipose and muscle tissues. An association analysis indicated that the incremental variable duplication was associated with body measurements. |
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| Keywords: | Adiponectin bovine growth traits mRNA expression promoter activity variation |
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