Oxidative biotransformation of thioanisole by <Emphasis Type="Italic">Rhodococcus rhodochrous</Emphasis> IEGM 66 cells |
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Authors: | A A El’kin V V Grishko I B Ivshina |
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Institution: | 1.Institute of Ecology and Genetics of Microorganisms, Ural Division,Russian Academy of Sciences,Perm,Russia;2.Institute of Technical Chemistry, Ural Division,Russian Academy of Sciences,Perm,Russia |
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Abstract: | Comparative study of sulfoxidation activity of free and immobilized Rhodococcus rhodochrous IEGM 66 cells was performed. Free Rhodococcus cells (in the presence of 0.1 vol % n-hexadecane) displayed maximal oxidative activity towards thioanisole (0.5 g/l), a prochiral organic sulfide, added after
48-h cultivation of bacterial cells. Higher sulfide concentrations inhibited sulfoxidation activity of Rhodococcus. Use of immobilized cells allowed the 2-day preparatory stage to be omitted and a complete thioanisole bioconversion to be
achieved in 24 h in the case that biocatalyst and 0.5 g/l thioanisole were added simultaneously. The biocatalyst immobilized
on gel provides for complete thioanisole transformation into (S)-thioanisole sulfoxide (optical purity of 82.1%) at high (1.0–1.5
g/l) concentrations of sulfide substrate. |
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