Involvement in Meiotic Prophase of H1 Histone Kinase and p34cdc2 Homologues in Lily (Lilium longiflorum) Microsporocytes |
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| Authors: | Akihiko Yamaguchi Masakane Yamashita Michiyasu Yoshikuni Yasuo Hotta Paul Nurse Yoshitaka Nagahama |
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| Affiliation: | Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444, Japan;Department of Biology, School of Science, Nagoya University, Nagoya 464, Japan;ICRF Cell Cycle Control Laboratory, Microbiology Unit, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, England |
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| Abstract: | We have taken advantage of the synchrony of meiotic prophase I in Lilium microsporocytes to investigate the presence and involvement in four stages of meiotic prophase I (leptotene, zygotene, pachytene, and diplotene) of the p34cdc2 H1 histone kinase, a component of MPF and a key participant in division control in other eukaryotes. H1 kinase activity showed a peak pattern during meiotic prophase I with the highest kinase activity at pachytene. A monoclonal antibody directed against a highly conserved region of p34cdc2 (termed the 'PSTAIR') recognized three major protein forms by immunoblotting. The highest level of the fastest-migrating form was observed at pachytene, coinciding with the highest activity of H1 kinase. Both the proteins recognized by the anti-PSTAIR antibody and H1 histone kinase activity were retained on beads conjugated with p13suc1, a protein known to physically associate with p34cdc2. These observations suggest that p34cdc2 or protein(s) highly homologous to p34cdc2 is a component of Lilium H1 histone kinase and plays a role in regulating meiotic prophase I. |
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