Electrochemical properties of hydrogenase on glass carbon electrodes modified with carbon nanotubes |
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Authors: | Ai-Rong Liu Dong-Jin Qian Meng Chen Tatsuki Wakayama Chikashi Nakamura Nikolay A. Zorin Jun Miyake |
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Affiliation: | (1) Department of Chemistry, Fudan University, 220 Handan Road, 200433 Shanghai, P.R. China;(2) Research Institute of Cell Engineering, AIST, Amagazaki 661-0974, Hyogo, Japan;(3) Institute of Basic Biological Problems, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia |
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Abstract: | Hydrogenase (H2 ase) purified from phototropic bacteriumThiocapsa roseopersicina was coassembled with carbon nanotubes (CNTs) on glass carbon electrodes. Both oxidized CNTs and Nafion-CNT composites were used to modify the electrodes. The pure H2 are formed dot-like domains, while the oxidized CNT-H2 ase and Nafion-CNT-H2 ase composites formed wire-like and large closely packed aggregates, respectively. The reductive potentials for the [4Fe-4S]2+/1+ clusters of H2ase were at about −500, −650, and −700 mV (vs Ag/AgCl) for the electrodes modified with pure H2ase, Nafion-SWNT-H2ase, and Nafion-MWNT-H2ase composites, respectively. Potential step chronocoulometry measurements indicated a larger charge-transfer diffusion coefficient between the H2ase and electrodes when the CNTs were co-assembled with H2ase, suggesting that the CNTs can not only act as a supporting layer to immobilize enzymes, but also act as a highly conductive wire throughout the films. |
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Keywords: | Hydrogenase electrochemistry carbon nanotubes chronocoulometry SEM |
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