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Characterization of the 3a protein of SARS-associated coronavirus in infected vero E6 cells and SARS patients |
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| Authors: | Zeng Rong Yang Rui-Fu Shi Mu-De Jiang Man-Rong Xie You-Hua Ruan Hong-Qiang Jiang Xiao-Sheng Shi Lv Zhou Hu Zhang Lei Wu Xiao-Dong Lin Ying Ji Yong-Yong Xiong Lei Jin Yan Dai Er-Hei Wang Xiao-Yi Si Bin-Ying Wang Jin Wang Hong-Xia Wang Cui-E Gan Yong-Hua Li Yu-Chuan Cao Ju-Tian Zuo Jiang-Ping Shan Shi-Fang Xie En Chen Song-Hua Jiang Zhi-Qin Zhang Xi Wang Yuan Pei Gang Sun Bing Wu Jia-Rui |
| Institution: | Research Center for Proteome Analysis, Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China. |
| Abstract: | Proteomics was used to identify a protein encoded by ORF 3a in a SARS-associated coronavirus (SARS-CoV). Immuno-blotting revealed that interchain disulfide bonds might be formed between this protein and the spike protein. ELISA indicated that sera from SARS patients have significant positive reactions with synthesized peptides derived from the 3a protein. These results are concordant with that of a spike protein-derived peptide. A tendency exists for co-mutation between the 3a protein and the spike protein of SARS-CoV isolates, suggesting that the function of the 3a protein correlates with the spike protein. Taken together, the 3a protein might be tightly correlated to the spike protein in the SARS-CoV functions. The 3a protein may serve as a new clinical marker or drug target for SARS treatment. |
| Keywords: | SARS coronavirus 3a protein spike protein proteome |
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