Sucrose and light effects on in vitro cultures of potato,chokecherry and saskatoon berry during low temperature storage |
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Authors: | Pruski Kris Kozai Toyoki Lewis Tina Astatkie Tess Nowak Jerzy |
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Institution: | (1) Crop Diversification Centre North, 17507-Fort Road, Edmonton, AB, Canada, T5B 4K3;(2) Department of Agricultural Sciences, Nova Scotia Agricultural College, P.O.Box 550, Truro, NS, Canada, B2N 5E3;(3) Faculty of Horticulture, Chiba University, Matsudo, Chiba 271, Japan;(4) Department of Engineering, Nova Scotia Agricultural College, P.O.Box 550, Truro, NS, Canada, B2N 5E3;(5) Department of Agricultural Sciences, Nova Scotia Agricultural College, P.O.Box 550, Truro, NS, Canada, B2N 5E3 |
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Abstract: | Cultures of potato (Solanum tuberosum) cv. Atlantic, chokecherry (Prunus virginiana L.) cv. Garrington and saskatoon berry (Amelancher alnifolia Nutt.) cv. Northline grown in vitro for 3 weeks at 24/22 °C, 16-h photoperiod, 150 μmol m−2 s−1 photosynthetic photon flux density (PPFD) mixed fluorescent/incandescent light were stored for 6, 9 and 12 weeks at 4 °C
under 0 (darkness) and 3 μmol m−2 s−1 PPFD (690 nm red light continuous illumination). Growth regulators free MSMO medium either with or without 30 g l−1 sucrose was used to store the cultures. All cultures retained capacity to re-grow after storage. Tested factors, sucrose,
light and the length of the storage period had an impact on shoot quality and re-growth capacity of the cultures. For either
light treatment sucrose was essential for the low temperature maintenance of vigorous stock plants of potato, if stored for
over 6 weeks. Chokecherry and saskatoon cultures stored well without sucrose; although chokecherry benefited from sucrose
in the storage medium when the stock cultures were kept at the low temperature for 12 weeks. Low light significantly improved
quality of the stored potato cultures, but had very little effect on both chokecherry and saskatoon berry cultures. The woody
plant cultures grew during storage, and the longer the stock plants were stored, the more vigorous cultures they generated.
The results indicate that growers can successfully use their existing facilities, small refrigerators and coolers with low
light intensity, set at 4 °C, for short term storage of potato, chokecherry and saskatoon berry cultures. The potato cultures,
which are known to be sensitive to prolonged low temperature storage, should be frequently monitored and subcultured as required.
On the other hand, the woody plant stock cultures do not require any special attention when kept at 4 °C and re-grow the most
vigorous shoots if stored for at least 12 weeks.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Amelanchier alnifolia Nutt capacity to re-grow in vitro storage Prunus virginiana L Solanum tuberosum L |
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