Skin unhairing proteases of Bacillus subtilis: production andpartial characterization |
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Authors: | Hermosinda Varela Mario Daniel Ferrari Leopoldo Belobrajdic Adriana Vazquez Milka Lyliam Loperena |
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Institution: | (1) Depto. Bioingeniería, Instituto de Ingeniería Química, Facultad de Ingeniería, Universidad de la República, J. Herrera y Reissig 565, 11300 Montevideo, Uruguay |
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Abstract: | Proteases able to unhair sheep skins were produced from Bacillus subtilis. Protease activity was increased from 640 to 990 U/mL by using a fed-batch culture with glucose added sequentially up to 10 g/L. The crude enzyme prep-aration was mainly a mixture of metallo- and serine-proteases with optimal pH and temperature for protease activity at 7 and 50-55°C, respectively. At pH 8 and 30°C, protease activity of the crude enzyme was 33% of the maximal value and 97% of the original activity (900 U/mL) was retained after incubation for 4 hours. |
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