Biotransformation studies using hairy root cultures - A review |
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Authors: | Banerjee Suchitra Singh Sailendra Ur Rahman Laiq |
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Institution: | Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants, (CIMAP-CSIR), Kukrail Picnic Spot Road, P.O. CIMAP, Lucknow 226015, India. suchitrabanerjee07@yahoo.com |
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Abstract: | Agrobacterium rhizogenes induced hairy root cultures are entering into a new juncture of functional research in generating pharmaceutical lead compounds by bringing about chemical transformations aided through its inherent enzyme resources. Rational utilization of hairy root cultures as highly effective biotransformation systems has come into existence in the last twenty years involving a wide range of plant systems as well as exogenous substrates and diverse chemical reactions. To date, hairy root cultures are preferred over plant cell/callus and suspension cultures as biocatalyst due to their genetic/biochemical stability, hormone-autotrophy, multi-enzyme biosynthetic potential mimicking that of the parent plants and relatively low-cost cultural requirements. The resultant biotransformed molecules, that are difficult to make by synthetic organic chemistry, can unearth notable practical efficacies by acquiring improved physico-chemical properties, bioavailability, lower toxicity and broader therapeutic properties. The present review summarizes the overall reported advances made in the area of hairy root mediated biotransformation of exogenous substrates with regard to their reaction types, plant systems associated, bacterial strains/molecules involved and final product recovery. |
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Keywords: | Biotransformation Exogenous substrate Hairy root cultures Reaction types Product recovery Plant systems |
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