PENG Fang-Ren银杏营养贮藏蛋白质的亚细胞定位(英文)

在电子显微镜下,对银杏(GinkgobilobaL.)枝条营养贮藏蛋白质的超微结构特征及在亚细胞水平的定位进行了系统研究。结果表明:银杏营养贮藏蛋白质主要存在于韧皮薄壁细胞的液泡内。银杏韧皮薄壁细胞内的营养贮藏蛋白质在细胞质内合成,由内质网膨大的槽库、质膜内折或高尔基体小泡发育形成贮藏蛋白质的液泡。液泡蛋白质主要以不定形块状、絮状或颗粒状形态存在。贮藏蛋白质在整个越冬期一直保持高含量,直到翌年春季萌芽时,贮藏蛋白质迅速转移再利用。随着新梢的生长,到了夏末秋初,又重新开始积累贮藏蛋白质。

Subcellular Localization of Vegetative Storage Protein of Ginkgo biloba

The ultrastructural characteristics and the subcellular localization of vegetative storageproteins (VSPs) of Ginkgo biloba L. were systematically studied under the electron microscope. Resultsindicated that the VSPs of G. biloba were mostly distributed in the small vacuoles of the phloemparenchyma cells. The VSPs of phloem parenchyma cells were produced in cytoplasm, then separated bythe inflated cisternae of endoplasmic reticulum (ER), plasmalemma invagination or Golgi body vesicles,resulting in the formation of vacuole filled with proteins. Three kinds of VSPs were detected: granular,floccular and massive VSPs, which were distributed in different tissue cells and different cells of the sametissue or vacuoles of the same cells. VSPs accumulated in autumn and kept at high level throughout thewinter. In the following spring, the bud started growing and VSPs were completely mobilized. With newshoots growth, VSPs resumed accumulating in late summer and early autumn.