Precipitating antibodies of the duck (Anas platyrhynchos) |
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Authors: | D A Higgins |
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Affiliation: | Department of Pathology, University of Hong Kong, Queen Mary Hospital. |
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Abstract: | 1. Ducks do not usually produce precipitating antibodies. However, when hyperimmunized with the dinitrophenyl (DNP)-human IgG (HIgG) conjugate, ducks made precipitating antibodies to the HIgG carrier, though not to the DNP hapten. 2. Precipitation did not require NaCl and occurred over a wide range of molarities and pH. 3. Antigenic and polyacrylamide gel analysis of affinity-purified antibodies, indicated that the major constituent of the antibody population was electrophoretically homogeneous 5.7S IgG. 4. The Ig heavy chains were in two populations, viz MW 37,000 and 41,000; non-precipitating duck 5.7S IgG antibodies to HIgG had only the MW 37,000 heavy chains. 5. It is suggested that duck precipitins belong to a previously unrecognized subclass of duck 5.7S IgG, with minor physical and antigenic differences from their non-precipitating counterparts. |
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