Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and L. pennellii |
| |
Authors: | M A O'Connell M R Hanson |
| |
Institution: | (1) Plant Genetic Engineering Laboratory and Agronomy and Horticulture Department, New Mexico State University, 88003 Las Cruces, NM, USA;(2) Section of Genetics and Development, Cornell University, Bradfield Hall, 14853 Ithaca, NY, USA |
| |
Abstract: | Summary Somatic hybrid plants have been regenerated following polyethylene glycol mediated fusion of leaf mesophyll protoplasts from tomato and protoplasts from Lycopersicon pennellii callus. Three different cultivars of tomato were used as sources of protoplasts: Early Girl, Manapal, and UC82B. Fusions were performed between protoplasts of these tomato cultivars and protoplasts of L. pennellii, and between protoplasts of the cultivars and protoplasts of L. pennellii that had been exposed to 3 or 6 krads of gamma radiation. Somatic hybrid plants were identified on the basis of heterozygous isozyme banding patterns, and leaf and flower morphology. Somatic hybrid plants were regenerated following fusion of tomato protoplasts with either untreated or irradiated L. pennellii protoplasts. All were heterozygous for isozyme loci on five different chromosomes. Regenerated somatic hybrids showed inheritance of either or both parental chloroplast genomes, but predominantly the L. pennellii mitochondrial genome. The regenerated somatic hybrid plants exhibited reduced fertility, less than 20% viable pollen. A total of 34 somatic hybrid calli were identified. Of these, 21 regenerated shoots, and 7 produced seed following manual pollinations. |
| |
Keywords: | Tomato Somatic hybrid Organelle genome Protoplast fusion Irradiation |
本文献已被 SpringerLink 等数据库收录! |
|