| 辣根过氧化物酶在水相胶束中的动力学 |
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| 引用本文： | 刘晓辉,刘伟,王金堆,周永亮,马林,付学奇,李铁津.辣根过氧化物酶在水相胶束中的动力学[J].中国生物化学与分子生物学报,1995,11(6):702-705. |
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| 作者姓名： | 刘晓辉 刘伟 王金堆 周永亮 马林 付学奇 李铁津 |
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| 作者单位： | 吉林大学分子生物学系，酶工程国家重点实验室 |
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| 摘 要： | 研究了辣根过氧化物酶在三种表面活性剂（ＳＤＳ，ＴｒｉｔｏｎＸ－１００及ＣＴＡＢ）的水相胶束中催化联苯胺聚合反应的动力学。结果表明水相胶束体系有利于反应的进行。辣根过氧化物酶在水相胶束体系中遵循米氏反应，Ｋ_ｍ在ＳＤＳ、ＴｒｉｔｏｎＸ－１００及ＣＴＡＢ三种体系中分别为３．０１４×１０~（－４）ｍｏｌ／Ｌ、１．７２８×１０~（－４）ｍｏｌ／Ｌ和５．６６４×１０~（－５）ｍｏｌ／Ｌ。由于微环境的不同，ＨＲＰ在三种体系中表现出不同的最适反应温度和最适ｐＨ。
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| 关 键 词： | 辣根过氧化物酶 水相胶束 联苯胺 |
| 收稿时间： | 1995-12-20 |
Kinetics of Horseradish Peroxidase in Aqueous Micelles |
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| Liu,Xiao-Hui,Liu,Wei,Wang,Jin-Dui,Zhou,Yong-Liang,Ma,Lin Fu,Xue-Qi,Li,Tie-Jin.Kinetics of Horseradish Peroxidase in Aqueous Micelles[J].Chinese Journal of Biochemistry and Molecular Biology,1995,11(6):702-705. |
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| Authors: | Liu Xiao-Hui Liu Wei Wang Jin-Dui Zhou Yong-Liang Ma Lin Fu Xue-Qi Li Tie-Jin |
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| Institution: | (Molecular Biology Department,Chemistry Department and National Lab of Enzyme Engineering,Jilin University,Changchun 130023 |
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| Abstract: | The kinetics of HRP oxidizing benzidine were investigated in three typical aqueous micellar systems which were formed by SDS, Triton X-100 and CTAB.The results indicated that the catalysis of HRP in these system followed Mechaelis-Menten mechanism as it did in the media of water and organic solvent. The measured K_m Values in SDS, Triton X-100 and CTAB micellar systems were 3.14 ×10￣(-4) mol/L, 1.728 ×10￣(-4)mol/L and 5.664 ×10￣(-5) mol/L, respectively.Moreover, HRP presents distinct optimal temperature and PH in these micellar systems , which may result from the disparity of microenvironment in each one. |
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| Keywords: | Horseradish peroxidase Aqueous micelle Benzidine |
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