| Abstract: | Two kinds of TBG polymorphism are described in human, one found in deglycosylated TBG from individual blood donors, the other is a genetically determined polymorphism. TBG from plasma samples from a patient with toxic goiter, not autoimmune, (p)TBG, from the patient's mother (m)TBG and from individual donors (n)TBG, were labeled with 125I]T4 or 125I]T3 and submitted to isoelectric focusing (IEF), followed by autoradiography. Three faint 125I]T4 radiolabeled bands were detectable in (p)TBG while four strong 125I]T4 radiolabeled bands were detectable in (m)TBG and (n)TBG), respectively. IEF of the 125I]T3 incubated serum samples resulted in no detectable isoelectric radiolabeled band for (p)TBG while a normal pattern was found in (m)TBG and in (n)TBG, respectively. These data suggest a new intraindividual not linked to sexual chromosome X polymorphism characterized by a loss in hormone binding. |
