Dissection of an old protein reveals a novel application: domain D of <Emphasis Type="Italic">Staphylococcus aureus</Emphasis> Protein A (sSpAD) as a secretion - tag |
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| Authors: | Thomas Heel Michael Paal Rainer Schneider Bernhard Auer |
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| Institution: | 1.Austrian Center of Industrial Biotechnology (ACIB),TU Graz,Graz,Austria;2.Institute of Biochemistry,University of Innsbruck,Innsbruck,Austria;3.Center for Molecular Biosciences (CMBI),University of Innsbruck,Innsbruck,Austria |
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| Abstract: | Background
Escherichia coli as a frequently utilized host organism for recombinant protein production offers different cellular locations with distinct
qualities. The periplasmic space is often favored for the production of complex proteins due to enhanced disulfide bond formation,
increased target product stability and simplified downstream processing. To direct proteins to the periplasmic space rather
small proteinaceus tags that can be used for affinity purification would be advantageous. |
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