Production of transgenic Pinus armandii plants harbouring btCryIII(A) gene |
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| Authors: | X. Z. Liu Z. Liu Y. M. Yang H. Y. Zhang |
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| Affiliation: | (1) Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People’s Republic of China;(2) College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, People’s Republic of China;(3) Graduate University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China; |
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| Abstract: | A synthetic chimeric gene SbtCryIII(A) encoding the insecticidal protein btCryIII(A), was transformed into Pinus armandii embryos and embryogenic calli using Agrobacterium tumefaciens. Polymerase chain reaction and genomic DNA Southern blot analysis showed that the SbtCryIII(A) gene was integrated into the genome of transgenic Pinus armandii plants, and Northern blot analysis indicated that the SbtCryIII(A) gene was transcribed. |
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